A51 CHONDROCYTEMMP-13 EXPRESSION IN RESPONSE TO ALPHA5BETA1 INTEGRIN STIMULATION REQUIRES PROTEIN SULFENIC ACIDS AS SIGNALING INTERMEDIATES

Protein sulfenic acids in redox signaling.

Reactive (low pKa) cysteine residues in proteins are critical components in redox signaling. A particularly reactive and versatile reversibly oxidized form of cysteine, the sulfenic acid (Cys-SOH), has important roles as a catalytic center in enzymes and as a sensor of oxidative and nitrosative stress in enzymes and transcriptional regulators. Depending on environment, sometimes the sulfenic ac...

Complex and dynamic redistribution of NF-kappaB signaling intermediates in response to T cell receptor stimulation.

The central zone of the supramolecular activation cluster (c-SMAC) is a zone of T cell receptor (TCR) enrichment that forms at a T cell/antigen-presenting cell (APC) junction in response to antigen stimulation. We demonstrate that there is a surprisingly complex relocalization process that brings PKC and Bcl10, two intermediates in TCR activation of NF-kappaB, to the cytoplasmic face of the c-S...

Response of laying hens to supplementation with various fatty acids when fed diets inadequate in protein and sulphur amino acids

A Cell-Permeable Biscyclooctyne As a Novel Probe for the Identification of Protein Sulfenic Acids.

Reactive oxygen species act as important second messengers in cell signaling and homeostasis through the oxidation of protein thiols. However, the dynamic nature of protein oxidation and the lack of sensitivity of existing molecular probes have hindered our understanding of such reactions; therefore, new tools are required to address these challenges. We designed a bifunctional variant of the s...

Changes in regulator of G protein signaling-4 gene expression in the spinal cord of adrenalectomized rats in response to intrathecal morphine

Introduction: Regulators of G-protein signaling protein negatively control G protein -coupled receptor signaling duration by accelerating Gα subunit guanosine triphosphate hydrolysis. Since regulator of G-protein signaling4 has an important role in modulating morphine effects at the cellular level and the exact mechanism(s) of adrenalectomy-induced morphine sensitization have not been fully cl...